Proteomic Analysis of Sputum from Patients with Active Tuberculosis.

ABSTRACT:

Background

Patients with pulmonary tuberculosis (TB) typically produce sputa, which are used to identify the pathogen. Sputum also contains host proteins that may aid in diagnosis. We hypothesized that sputa from TB patients will have unique proteomes when compared to other lung diseases.

Methods

Sputa were collected from 219 patients with suspected TB. Neutrophil-derived protein calprotectin (CP), which was used as a marker for lung damage, was quantified and compared between TB and non-TB groups. Three sputa with high or low CP from each group were selected and analyzed using label-free proteomics.

Results

There was no difference in CP amounts between TB and non-TB groups. However, TB samples had other differentially abundant neutrophil-associated proteins. Compared

SUBMITTER: Marcantonio E 

PROVIDER: S-EPMC12452556 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature