Cost-Effective Method for Full-Length Sequencing of Monoclonal Antibodies from Hybridoma Cells.

ABSTRACT:

Background

Monoclonal antibodies play an important role in therapeutic and analytical applications. For recombinant expression, the coding sequences of the variable regions of the heavy and light chains are required. In addition, cloning antibody sequences, including constant regions, reduces the impact of hybridoma cell loss and ensures preservation of the naturally occurring full antibody sequence.

Method

We combined amplification of IgG antibody variable regions from hybridoma mRNA with an advanced method for full-length cloning of monoclonal antibodies in a simple two-step workflow. Following Sanger sequencing and evaluation of consensus sequences, the best matching variable, diversity, and joining (V-(D-)J) gene segments were identified according to identity scores from

SUBMITTER: Doring S 

PROVIDER: S-EPMC12452578 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature