Isolation, Extraction, and Analysis of Cells After Confined Migration.

ABSTRACT: Cell migration through confined microenvironments is a critical biological process that underlies numerous physiological and pathological events, including immune cell trafficking, tissue morphogenesis, and cancer metastasis. Although polydimethylsiloxane-based microchannel devices have enabled detailed studies of confined migration, the efficient collection of cells post-migration for downstream molecular analyses remains a major challenge. Existing approaches often rely on harsh mechanical dissociation that compromises cell viability and integrity and do not permit in situ collection of cell lysates. To overcome these limitations, we have developed the Trap-based Recovery After Permeation (TRAP) chip, a pump-free microfluidic platform that integrates controlled confined migration with ef

SUBMITTER: Gao X 

PROVIDER: S-EPMC12452805 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature