Surface protein glycosylation conserved in the human pathogen Mycoplasma genitalium and retained in the synthetic organism JCVI-Syn3A.

ABSTRACT: Protein glycosylation has been reported in all forms of life. The genus Mycoplasma is composed of highly genome-streamlined bacterial symbionts, making them model organisms for investigating minimal genome concepts. Previous work from our group showed mycoplasmas scavenge hexoses from exogenous oligosaccharides to glycosylate surface proteins at serine, threonine, asparagine, and glutamine residues without utilizing a consensus sequence as seen in canonical glycosylation systems. We report here that this surface protein hexosylation system is conserved in Mycoplasma genitalium, a human urogenital pathogen with a 580-kbp genome that can be cultured axenically. We also report this modification is found in the ruminant pathogen Mycoplasma mycoides subsp. capri and is conserved in JCVI-Syn3A,

SUBMITTER: Sanford JW 

PROVIDER: S-EPMC12453214 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature