Application of an imaging flow cytometry γ-H2AX assay for biodosimetry using supervised machine learning.

ABSTRACT:

Purpose

Phosphorylation of the histone H2AX (γ-H2AX) is a rapid response to radiation-induced DNA double strand breaks (DSBs) and is a good biomarker for exposure to ionizing radiation. The signal has traditionally been detected by microscopy (spot counting) or by flow cytometry (fluorescent intensity). An imaging flow cytometry (IFC) method has been developed, which combines the high resolution of microscopy with the statistical power of flow cytometry methods to measure γ-H2AX in human lymphocytes.

Materials and methods

The assay was optimized and validated for both sample acquisition and data analysis, in the dose range of 0-10 Gy. For data analysis, mean fluorescence intensity (MFI), spot count (foci per cell), and average area of the spots were used with the supervised

SUBMITTER: Hassan EM 

PROVIDER: S-EPMC12453548 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature