Proteome-Wide Identification of <i>O</i>⁶-Methyl-2'-deoxyguanosine-Binding Proteins.

ABSTRACT: DNA is subjected to damage from various endogenous and exogenous sources of alkylating agents, resulting in alkylated DNA lesions. Among these lesions, O⁶-methyl-2'-deoxyguanosine (O⁶-Me-dG) is highly mutagenic, and it can be repaired by O⁶-alkylguanine DNA alkyltransferase and mismatch repair pathway. It, however, remains unclear whether O⁶-Me-dG in DNA can be recognized by other cellular proteins. Here, we employed a quantitative mass spectrometry-based approach to uncover reader proteins of O⁶-Me-dG in DNA when it is paired with a 2'-deoxycytidine (dC) or thymidine (dT). We were able to identify 67 and 31 candidate reader proteins for duplex DNA harboring O⁶-Me-dG:dC and O<

SUBMITTER: Kellum AH 

PROVIDER: S-EPMC12453550 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature