An Optimized LIVE/DEAD Assay Coupled with Flow Cytometry for Quantifying Post-Stress Survival in Yeast Cells.

ABSTRACT: Quantifying survival is a common and critical task in yeast research. LIVE/DEAD stains based on plasma membrane integrity provide a rapid and high-throughput assay for yeast survival when coupled with flow cytometry. However, variations in staining buffer, dye concentration, incubation time, and flow cytometry settings can impact data quality and reproducibility. This protocol presents a standardized LIVE/DEAD assay for post-stress survival quantification in yeast using flow cytometry. After treating Candida glabrata, an opportunistic yeast pathogen, with different doses of hydrogen peroxide, the post-stress samples were stained with a two-component LIVE/DEAD stain consisting of SYTO 9 and propidium iodide (PI). Flow cytometry was used to distinguish live, damaged, and dead cell population

SUBMITTER: Tang H 

PROVIDER: S-EPMC12453624 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature