Characterization of acupuncture on central amino acid metabolism based on targeted neurotransmitter analysis in mice with inflammatory pain.

ABSTRACT:

Objective

To investigate the effect of central amino acid metabolic profiles on acupuncture analgesia.

Methods

BALB/c mice were injected with Complete Freund's Adjuvant (CFA) and acupuncture was applied at Zusanli (ST36) for 7 d after modeling. Paw withdrawal thermal latency (PWTL) and paw withdrawal mechanical threshold (PWMT) were tested during the experiment. The level of prostaglandin E2 (PGE2) was measured by enzyme-linked immunosorbent assay (ELISA). Using liquid chromatography-mass spectrometry (LC-MS/MS) based on the multiple reaction monitoring (MRM)-targeted metabolomic approach, neurotransmitter in the brain and spinal cord as well as were detected, further tryptophan metabolites in spinal cord were detected, and the data was analyzed using multiquant software.

SUBMITTER: Yue W 

PROVIDER: S-EPMC12453991 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature