Circadian clock activity in human umbilical vein endothelial cells of preterm and term neonates.

ABSTRACT:

Background

During mammalian gestation, fetal circadian rhythms are thought to be mainly controlled by maternal signals. In humans, the initiation and activity of central and peripheral circadian clocks is largely unknown. This study aimed to elucidate the developmental clock properties in human umbilical vein endothelial cells (HUVECs).

Methods

HUVECs were obtained from (a) preterm infants, subgrouped according to birth weight or gestational age classification, and (b) term infants (in total: n = 60). In vitro clock activity was determined by using live bioluminescence recording of a luciferase reporter gene under circadian control over 120 h. In addition, core clock and clock-associated gene expression were quantified using NanoString technology.

Results

Peripheral c

SUBMITTER: Zuo M 

PROVIDER: S-EPMC12454134 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature