Prolonged early-life antibiotic exposure alters gut microbiota but does not exacerbate lung injury in a rat pup model.

ABSTRACT:

Background

Early antibiotic exposure may disrupt gut microbiome and affect the gut-lung axis. We examined the impact of prolonged antibiotic exposure during early life on growth and subsequent acute lung injury (ALI) in a rat pup model.

Methods

Thirty-four 7-day-old rat pups were divided into Control, Antibiotics (Anti), Lung injury (LI), and Antibiotics-Lung Injury (Anti-LI) groups. The Anti and Anti-LI groups received oral Amoxicillin-Clavulanic acid from 7 to 40 days old, while Control and LI groups received sham water. ALI was induced in LI and Anti-LI groups with intratracheally administered lipopolysaccharide at 41 days old; all were sacrificed at 42 days old. Fecal bacterial sequencing, serum cytokine analysis, and pulmonary histological examination were performed.

SUBMITTER: Hsueh MY 

PROVIDER: S-EPMC12454151 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature