Membrane-targeting antibacterial isoniazid schiff base against <i>S. aureus</i> and biofilms.

ABSTRACT:

Introduction

Building upon previous research, this study focuses on the replication and evaluation of a series of hydrazone derivatives derived from isoniazid.

Methods

The lead compound, identified as C5, was assessed for its antibacterial activity against Gram-positive bacteria, notably Staphylococcus aureus ATCC 29213. Its hemolytic potential, cytotoxicity (against VERO cells), and ability to induce resistance were evaluated. Mechanistic studies included assays for membrane depolarization (using DiSC35 fluorescence), membrane integrity (via SYTOX Green uptake), measurement of intracellular ATP levels, and detection of reactive oxygen species (ROS). Additional investigations examined its effect on LPS-induced NO/TNF-α release in macrophages and its activity against S. au

SUBMITTER: Liu Y 

PROVIDER: S-EPMC12454367 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature