Establishing essential oil stewardship through the case of rosemary and thyme oils against <i>Staphylococcus aureus</i>.

ABSTRACT: Essential oils (EOs) have long been studied for their antimicrobial properties, yet most investigations rely on simplistic models, limited strain panels, and anecdotal interpretations-failing to meet the standards expected of modern anti-infective agents. Advancing beyond this tradition, we implement a framework developed over several years of systematic investigation. Using this approach, we assessed the antibiofilm activity of Rosmarinus officinalis L. and Thymus vulgaris L. EOs against a panel of clinical Staphylococcus aureus isolates obtained from non-healing wounds. By applying infection-relevant conditions, such as wound-mimicking media and surfaces, strain-level resolution, and both contact and volatile exposure, we revealed substantial inter-strain variability

SUBMITTER: Brozyna M 

PROVIDER: S-EPMC12454425 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature