Tumor-informed circulating tumor DNA detection for personalized monitoring of treatment response in epithelial ovarian cancer.

ABSTRACT:

Background

Circulating tumor DNA (ctDNA) has emerged as a valuable biomarker in liquid biopsies for monitoring treatment responses in cancer patients. However, detecting ctDNA in epithelial ovarian cancer (EOC) is challenging due to its high heterogeneity and the absence of hotspot driver mutations. Therefore, a personalized approach to ctDNA analysis is essential, tailored to the specific tumor mutations of each EOC patient. In this study, we aimed to evaluate a droplet digital PCR (ddPCR) method targeting various genetic alterations in ctDNA identified through a targeted next-generation sequencing (NGS) panel in EOC tumors.

Methods

EOC tumor tissues were sequenced using a targeted NGS panel to identify oncogenic mutations. ddPCR assays were subsequently designed and optimi

SUBMITTER: Kang SW 

PROVIDER: S-EPMC12454446 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature