Benzo[a]pyrene and UV light co-exposure: differential effects on oxidative stress and genotoxicity in human keratinocytes and ex vivo skin.

ABSTRACT: Human skin exposure to environmental/occupational UV irradiation and polycyclic aromatic hydrocarbons (PAHs) is a concern. This study investigated the impact of the combined exposure on metabolism, cytotoxicity/viability, oxidative stress, and genotoxicity in human skin models. To understand the dose-response relationship, we exposed our models to a range of benzo[a]pyrene (B[a]P) concentrations. For the combined exposure scenario, the models were treated with B[a]P followed by UV irradiation. Furthermore, we employed two in vitro models: human keratinocytes and human skin explants. This allowed us to assess potential model-dependent variations in the observed toxicological effects. Keratinocytes displayed a partially synergistic response in most measured parameters at 0.4 µM B[a]P or high

SUBMITTER: Kersch C 

PROVIDER: S-EPMC12454581 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature