The role of microglia and complement C5/C5a in the pathogenesis of rhegmatogenous retinal detachment with choroidal detachment.

ABSTRACT:

Background

Rhegmatogenous retinal detachment with choroidal detachment (RRDCD) is an uncommon and sight-threatening disorder marked by fast development and significant inflammation. This study aimed to identify cellular and molecular signatures distinguishing RRDCD from typical rhegmatogenous retinal detachment (RRD) and to investigate the roles of microglia and the complement C5/C5a pathway in disease pathogenesis.

Methods

Single-cell RNA sequencing (scRNA-seq) was employed to analyze vitreous samples from patients with RRD and RRDCD to characterize the cellular composition and molecular pathways. In vitro co-culture experiments were performed to investigate the functional impact of complement C5 on primary retinal microglia, and their subsequent effects on RF/6 A endotheli

SUBMITTER: Xu H 

PROVIDER: S-EPMC12454608 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature