A hair-follicle reconstructed in vitro immunocompetent skin model for prediction of the sensitizing potential of chemicals.

ABSTRACT: The development of immunocompetent skin models represents a significant advancement in in vitro methods for detecting skin sensitizers, adhering to the 3R principles aimed at reducing, refining and replacing animal testing. In the present study, an advanced skin model from hair follicle-derived cells was constructed and enriched with two key immune cell types, namely Langerhans cells and T-lymphocytes, named ImmuSkin-MT. The model features a physiologically relevant epidermis and dermis, integration of monocyte-derived Langerhans cells (MoLCs) beneath the dermal layer, and co-cultivation with CD4⁺-T cells in the lower chamber of a transwell system. This setup closely mimics the native interplay between skin-resident immune cells and T-cells, marking a significant advancement in

SUBMITTER: Tripetchr T 

PROVIDER: S-EPMC12454623 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature