Monitoring ferroptosis in vivo: Iron-driven volatile oxidized lipids as breath biomarkers.

ABSTRACT: Ferroptosis, an iron-dependent cell death mechanism characterized by excessive lipid peroxidation, has been implicated in numerous human diseases and organ pathologies. However, current detection methods necessitate invasive tissue sampling to assess lipid peroxidation, making noninvasive detection of ferroptosis in human subjects extremely challenging. In this study, we employed oxidative volatolomics to comprehensively characterize the volatile oxidized lipids (VOLs) produced during ferroptosis. Polyunsaturated fatty acid-derived VOLs were generated via iron-dependent LPO and released extracellularly as ferroptosis progressed. These VOLs were specifically generated during hepatic ferroptosis in mouse models of acetaminophen-induced liver injury and metabolic dysfunction-associated steato

SUBMITTER: Matsuoka Y 

PROVIDER: S-EPMC12454666 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature