Identification of post-transcriptional regulation reveals complexity in peanut pod development by Direct RNA.

ABSTRACT: Peanut (Arachis hypogaea) is widely cultivated worldwide as an important source of edible vegetable oil and protein. Peanut seed pods develop below ground from a gynophore that forms above ground and then penetrates the soil surface to bury the developing pod. Numerous studies have explored transcriptional regulation during peanut pod development. Here, we explored post-transcriptional regulation, including polyadenylation, alternative splicing, and RNA adenosine methylation (m⁶A), in peanut pods across four developmental stages by performing direct RNA sequencing. This produced 70.43 million long reads with average lengths of 890-1,136 nucleotides (nt) from 12 samples across four developmental stages, yielding a total of 14,627 newly identified transcripts. We detected a

SUBMITTER: Wang W 

PROVIDER: S-EPMC12454814 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature