Predictable Modulation of a Spontaneous Post-translational Modification in Living Cells.

ABSTRACT: Glycation is a nonenzymatic posttranslational modification associated with aging and disease. Because it occurs spontaneously, it is extremely difficult to control the extent of glycation at distinct sites within target proteins, especially in cellular systems. Here, we report a chemical approach, referred to as "dialAGE", that enables the site-specific control of protein glycation. This unique tool requires the introduction of just a single point mutation that modulates the glycation susceptibility of a nearby arginine. As proof-of-concept, extensive mass spectrometry analysis was performed to confirm that dialAGE can modulate site-specific glycation levels at multiple arginine residues in ubiquitin in vitro, enabling both enhanced and diminished glycation. Introduction of dialAGE point m

SUBMITTER: Martin MS 

PROVIDER: S-EPMC12455423 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature