Metabolic Engineering of the 5-Aminolevulinate Biosynthetic Pathway in E. coli Improves Efficiency of Hemoprotein-Based Biocatalysis.

ABSTRACT: Biocatalysis using heme-dependent enzymes provides a powerful synthetic platform to facilitate a variety of chemical transformations required for organic synthesis. Despite recent advances in biocatalysis, recombinant expression systems for hemoproteins leave much room for improvement due to the strict regulation of heme biosynthesis in the host organism. To develop an efficient cofactor supplementation system for the expression of active holohemoproteins, we describe metabolic engineering of the heme biosynthetic pathway in E. coli. Through incorporation of a heterogeneous C4 pathway involving 5-aminolevulinic acid synthase of Paracoccus denitrificans, it was found that the concentrations of 5-aminolevulinic acid and heme in the engineered cells are increased during cultivation, and the e

SUBMITTER: Kato S 

PROVIDER: S-EPMC12455441 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature