A methyl-TROSY based ¹³C relaxation dispersion NMR experiment for studies of chemical exchange in proteins.

ABSTRACT: A methyl Transverse Relaxation Optimized Spectroscopy (methyl-TROSY) based, multiple quantum (MQ) ¹³C Carr-Purcell-Meiboom-Gill (CPMG) relaxation dispersion NMR experiment is described. The experiment is derived from the previously developed MQ ¹³C-¹H CPMG scheme (Korzhnev in J Am Chem Soc 126: 3964-73, 2004) supplemented with a CPMG train of refocusing ¹H pulses applied with constant frequency and synchronized with the ¹³C CPMG pulse train. The optimal ¹H 'decoupling' scheme that minimizes the amount of fast-relaxing methyl MQ magnetization present during CPMG intervals, makes use of an XY-4 phase cycling of the refocusing composite ¹H pulses. For small-to-medium sized proteins, the MQ ¹³C CPMG experim

SUBMITTER: Tugarinov V 

PROVIDER: S-EPMC12455490 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature