MicroRNAs Regulate Cellular Magnesium by Tuning Expression of the Plasma Membrane Protein CNNM4.

ABSTRACT: Cyclin M4 (CNNM4) is a plasma membrane Mg²⁺ transporter that is important for the regulation of cellular and organismal Mg²⁺ homeostasis. CNNM4 is overexpressed in liver diseases, including non-alcoholic steatohepatitis and acetaminophen-induced liver injury, leading to aberrant levels of Mg²⁺. The regulatory mechanisms underlying the overexpression of this protein, however, remain unknown. Here, we demonstrate regulation of CNNM4 and cellular magnesium levels by microRNA. Using a high-throughput assay, we established the complete miRNA binding profile of the 3'UTR of the gene. Both upregulation and downregulation of CNNM4 were observed, though upregulatory activity appears more prominent. We demonstrate that in both cases, the effects arise from direct bin

SUBMITTER: Lazarou TS 

PROVIDER: S-EPMC12455565 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature