Distinct circulating monocyte profiles in chronic cannabis users compared to cocaine users without changes in plasma levels of proinflammatory cytokines and LPS.

ABSTRACT:

Objectives

Chronic cannabis use is linked to anti-inflammatory effects, and cocaine exhibits context-dependent immunomodulation; their distinct impacts on monocyte subsets and systemic inflammation are not fully understood. Systemic microbial translocation contributes to monocyte differentiation, but the levels in chronic cocaine and cannabis users in humans in vivo are lacking.

Methods

Peripheral blood mononuclear cells (PBMCs) and plasma samples were collected from chronic cocaine users, cannabis users, and non-drug users. The route of drug administration was via smoking or snorting. Monocyte subsets were analyzed using flow cytometry; plasma levels of cytokines (IL-2, IL-4, IL-6, IL-10, TNF-α, and IFN-γ) and lipopolysaccharide (LPS, a marker of microbial translocat

SUBMITTER: Johnson D 

PROVIDER: S-EPMC12455578 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature