Spatiotemporal dynamics and selectivity of mRNA translation during mouse pre-implantation development.

ABSTRACT: Translational regulation plays a pivotal role during pre-implantation development. However, the mechanisms by which messenger RNAs (mRNAs) are selectively regulated over time, along with their dynamic utilization and fate during this period, remain largely unknown. Here, we performed fraction-resolved polysome profiling and characterized translational dynamics across oocytes and early embryo development. This approach allowed us to examine the changes in translation during pre-implantation development in high resolution and uncover previously unrecognized modes of translational selectivity. We observed a stage-specific delay in translation, characterized by the postponed recruitment of stored mRNAs-either unbound or associated with light ribosomal fractions-into actively translating polyso

SUBMITTER: Ming H 

PROVIDER: S-EPMC12455612 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature