YTK Display-and-Secrete: Screening for Optimal Protein Secretion Elements in <i>Saccharomyces cerevisiae</i>.

ABSTRACT: Engineering yeast to secrete target proteins requires searching for optimal combinations of promoters and signal peptides so that genes can be composed that give a high expression and efficient secretion. Most methods for this involve laborious, one-by-one assessments or require the use of enzymatic reporter proteins in order to achieve high-throughput capacity. Here, we introduce a novel modular method for the high throughput screening of yeast strains designed to secrete proteins of interest. Our approach integrates combinatorial DNA assembly, yeast surface display, flow cytometry, and nanopore DNA sequencing to facilitate rapid screening. Building on a widely used yeast toolkit (YTK) for modular cloning, our system creates surface display libraries with N- and C-terminal epitope tags by

SUBMITTER: Kishkevich A 

PROVIDER: S-EPMC12455636 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature