Directed Evolution of Branched-Chain α-Keto Acid Decarboxylase for 3-Hydroxypropionic Acid Production in <i>Escherichia coli</i> via Oxaloacetate.

ABSTRACT: 3-Hydroxypropionic acid (3-HP) serves as a crucial platform chemical with diverse applications across various industries. In this study, the oxaloacetate pathway was utilized for 3-HP production. This pathway involves the decarboxylation of oxaloacetate into malonic semialdehyde, catalyzed by branched-chain α-keto acid decarboxylase (KdcA), which is subsequently reduced to 3-HP by dehydrogenases. Directed evolution of KdcA was carried out to enhance its catalytic efficiency toward oxaloacetate, resulting in a KdcA^M8 mutant with the following substitutions: S286R, S287T, F381H, F382P, L534S, L535F, M538T, and G539F. Compared to wild-type (WT) KdcA, KdcA^M8 exhibits a lower K_M value toward oxaloacetate (K_M = 1.15 mM vs K_M</

SUBMITTER: Wang C 

PROVIDER: S-EPMC12455653 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature