Proteomic analysis and functional characterization of excretory-secretory products from adult Toxocara canis: insights into parasite-host interactions.

ABSTRACT:

Background

Toxocara canis is a globally distributed zoonotic nematode with significant public health implications. While previous studies have explored the excretory-secretory (ES) proteins of third-stage larvae (L3) and the non-protein components of adult excretory-secretory products (ESPs), a comprehensive molecular characterization of ES proteins from adult T. canis remains lacking.

Methods

ES proteins from female and male adult T. canis were profiled using an Orbitrap Astral mass spectrometer. Proteins with differential abundance (PDAs) between sexes were analyzed for subcellular localization, transcription factor, conserved domain, GO term and KEGG pathway enrichment. Immune-interacting ES proteins were identified via co-immunoprecipitation (Co-IP) followed by mass spec

SUBMITTER: Zhou YJ 

PROVIDER: S-EPMC12455767 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature