Development of a highly degenerate primer-based molecular tool for detecting and classifying the four major classes of polyhydroxyalkanoate synthase (phaC) genes in bacteria.

ABSTRACT: Polyhydroxyalkanoate synthase (phaC) gene encodes for PHA synthase enzyme which plays a key role in PHA polymerisation. To screen, unknown bacterial strains for their potential to produce PHAs, the presence of phaC gene is essential. Currently published primer sets targeting phaC gene are inadequate and often work only for well-studied genera (e.g., Pseudomonas, Cupriavidus). Few studies validate them in vitro, even fewer use degenerate primers to address phaC sequence diversity, and many fail to target all four phaC classes. In this study, nine novel highly degenerate primers were designed using the HYDEN (HighlY DEgeNerate) tool. The design included 65 phaC gene sequences from class I, 10 from class II, 19 from class III, 30 from class III/IV, and 6 from class IV, carefully selected as a

SUBMITTER: Ibrahim Osman A 

PROVIDER: S-EPMC12455824 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature