Functional orthogonality of parvoviral phospholipase A₂ domains in adeno-associated virus transduction.

ABSTRACT: Dependoparvoviruses, which belong to the family Parvoviridae, are being developed as viral vectors for gene transfer. Notably, different adeno-associated viral (AAV) serotype capsids have been utilized to generate pseudotyped recombinant vectors. While capsid surface regions mediate host cell interactions, buried structural domains have been implicated in parvoviral infectivity and post-entry trafficking. In this regard, the functional diversity of highly conserved group XIII phospholipase A₂ domains (PLA₂) located within the N-terminal capsid domain of different parvoviruses is of particular interest. Here, we developed a massively parallel screen to evaluate a diverse panel of rationally engineered and naturally derived parvoviral PLA₂ domains inco

SUBMITTER: Hull JA 

PROVIDER: S-EPMC12455919 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature