LEF-6 phosphorylation regulates the binding and trafficking of baculovirus late gene transcripts.

ABSTRACT: The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) LEF-6 has been shown to accelerate the infection cycle of AcMNPV, but its specific function is yet to be defined. In the present study, we identify LEF-6 as an RNA-binding protein that preferentially interacts with viral late transcripts. We demonstrate that the N-terminal half of LEF-6, which contains two typical ribonucleoprotein (RNP) motifs, is responsible for RNA binding, but its C-terminal half is required for the binding specificity to viral RNAs and for fulfilling its function in promoting virus infection. Further investigations reveal that LEF-6 has eight phosphorylated sites, and seven of them are located in the C-terminal half of LEF-6. The phosphorylation of LEF-6, especially the modification on the key site of S

SUBMITTER: Zhang X 

PROVIDER: S-EPMC12455930 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature