Cultivation and sequencing of microbiota members unveil the functional potential of yak gut microbiota.

ABSTRACT: The animal gut microbiota exhibits extensive taxonomic diversity, yet cultivated isolates and complete genomes from animal hosts remain scarce, hindering functional and ecological insights. We present a cultivated Yak (Bos grunniens) Fecal bacteria genome Reference (YFR), comprising 548 high-quality genomes based on aerobic and anaerobic cultivation. Notably, 216 strains represented novel taxa, classified into 29 species-level clusters spanning 4 phyla and 14 genera. The YFR increased the proportion of cultured ruminant gut bacterial species by 19.39%, significantly expanding the reference database for this ecosystem. Among these, 11 species harbor abundant CAZymes Gene Clusters (CGCs), indicating a high capacity for digesting complex polysaccharides. Biosynthetic Gene Clusters (BGC

SUBMITTER: Dai M 

PROVIDER: S-EPMC12455938 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature