ARRDC1 inhibits the replication of Semliki Forest virus by regulating the ubiquitination and degradation of viral nsP4.

ABSTRACT: Alphavirus infection can result in a spectrum of clinical manifestations in the host, including fever, rash, arthritis, and even symptoms of encephalitis, thereby posing a severe threat to global public health security. In this study, we explored the role of arrestin domain-containing protein 1 (ARRDC1) in the replication of Semliki Forest virus (SFV), an important member of alphaviruses, by siRNA-based knockdown or CRISPR/Cas9-mediated knockout techniques. SFV replication levels are significantly increased by knockdown or knockout of ARRDC1 in multiple cell lines and inhibited by trans-complementation with ARRDC1. Our data further revealed that ARRDC1 affects the early RNA replication stage of SFV. The antiviral effect of ARRDC1 is dependent on its cell plasma membrane localization and ub

SUBMITTER: Peng Q 

PROVIDER: S-EPMC12455954 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature