Aspartate potentiates tobramycin against multidrug-resistant <i>Edwardsiella tarda</i> through enhancing proton motive force and membrane permeability.

ABSTRACT: The antibiotic susceptibility/resistance of bacteria is influenced by their metabolic states. In tobramycin-resistant Edwardsiella tarda (LTB4-R_TOB), a dynamic metabolic state is observed, characterized by a general reduction in most metabolites as the minimum inhibitory concentration increases. Among these, aspartate downregulation emerges as the most critical biomarker. Exogenous aspartate enhances the efficacy of tobramycin, enabling it to effectively kill both lab-evolved and clinically isolated multidrug-resistant (MDR) E. tarda, as demonstrated in an animal model. Aspartate increases intracellular tobramycin levels in a concentration- and time-dependent manner by promoting drug uptake. Metabolic reprogramming analysis of LTB4-R_TOB and clinically is

SUBMITTER: Zeng Y-y 

PROVIDER: S-EPMC12456000 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature