Cathepsin L and transmembrane serine protease 11E mediate trypsin-independent entry of porcine deltacoronavirus into Huh7 cells.

ABSTRACT: Porcine deltacoronavirus (PDCoV) is an emerging enteric coronavirus with zoonotic potential. Previous studies showed that PDCoV productive infection is dependent on exogenous trypsin in porcine-derived cells. In this study, we found that trypsin appears to be dispensable for PDCoV infection in human-derived cell lines. Using Huh7 cells as a model, we investigated the potential mechanisms underlying this phenomenon. Our results demonstrated that exogenous trypsin has no significant impact on PDCoV proliferation in human-derived cells. Furthermore, the culture supernatants collected from Huh7 cells can promote PDCoV infection in porcine-derived cells under trypsin-free conditions. By utilizing protease inhibitors, we identified that PDCoV enters Huh7 cells via endosomal and plasma membrane f

SUBMITTER: Xiao W 

PROVIDER: S-EPMC12456011 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature