Multi-omics analysis reveals the genetic basis for rapid CO₂ utilization in the acetogenic bacterium <i>Sporomusa sphaeroides</i> KIAC.

ABSTRACT: Sporomusa sphaeroides KIAC is a novel acetogen isolated from cattle feces that exhibits rapid CO₂ utilization. To investigate the molecular basis of this phenotype, we performed a comprehensive multi-omics analysis, including Genome-seq, RNA-seq, dRNA-seq, and Term-seq, to map its transcriptome architecture. We identified 2,158 transcription start sites and 2,275 transcript 3' ends, enabling high-resolution reconstruction of the transcriptional landscape and associated regulatory features. This analysis uncovered key cis-regulatory elements and an expanded regulatory role for the alternative sigma factor SigH in controlling acetogenesis-related genes. Notably, KIAC harbors nine functionally diverse hydrogenases-a greater diversity than observed in other acetogens-l

SUBMITTER: Bae J 

PROVIDER: S-EPMC12456013 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature