CD103⁺CD8⁺ tissue-resident memory T lymphocytes of melanoma boost anti-tumour immunity and predict immunotherapy outcomes.

ABSTRACT:

Background

Immunotherapy has revolutionised melanoma treatment, providing significant clinical benefits by reactivating the anti-tumour immune system. CD8⁺ tissue-resident memory T lymphocytes (CD8⁺ TRM) have emerged as crucial mediators of anti-tumour immunity, while their specific role in melanoma remains poorly understood.

Methods

Following CD8⁺CD45.1⁺ OT-1 cell adoptive transfer into CD45.2⁺ mice, we employed magnetic separation to purify and analyse resident memory CD8⁺ T cells (TRM). We use multiple immunohistochemistry (mIHC) to evaluate the spatial distribution of CD8⁺ TRM in ZS melanoma cohort. Additionally, the biological function of CD8⁺ TRM and their impact on anti-tumour immu

SUBMITTER: Zhang T 

PROVIDER: S-EPMC12456097 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature