Visualization and tracking of tubule-derived, fluorescent-labeled NS1 as a marker of bluetongue virus in living cells.

ABSTRACT: Bluetongue virus (BTV) is an arbovirus (genus, Orbivirus; family, Sedoreoviridae) transmitted among ruminant hosts by Culicoides biting midges (genus, Culicoides). Orbivirus sp. produce large numbers of virus-specific tubules during infection. Non-structural protein 1 (NS1) derived from BTV is a highly conserved tubule-forming protein that exists in infected cells as tubular and non-tubular forms. However, the lack of tagged viruses has limited dynamic tracking of NS1 tubule protein levels in living cells. Here, four recombinant viruses (BTV1-NS1-156TC, BTV1-NS1-156eGFP, BTV1-NS1-534eGFP, and BTV1-NS1-552eGFP) were successfully rescued using a BTV reverse genetics strategy by introduction of biarsenical-tetracysteine or enhanced green fluorescent protein

SUBMITTER: Song Y 

PROVIDER: S-EPMC12456127 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature