3D imaging and quantitative analysis of adipocytes in situ and ex situ.

ABSTRACT: Different adipose tissues (AT) have been described, including subcutaneous and visceral tissues (SCAT and VAT). They display different morphological structures, physiological and metabolic functions. Imaging adipocytes in the whole AT was not feasible because of the large adipocyte sizes and the lipid-full content of the droplets that increased the refractive index. Tissue clearing is then required mainly through a delipidation step, which induces also a tissue shrinkage. Our aim was to image in 3D freshly extracted adipocytes and compare them to those within their tissues. Trout ATs were stained with 5DTAF (extracellular matrix) and Nile Red (lipids). After clearing with Histodenz, 3D images were obtained using a confocal microscope, and adipocytes were segmented and measured. In situ, ma

SUBMITTER: Hue I 

PROVIDER: S-EPMC12456214 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature