Extracellular Vesicles in Serum Carry Trop2 Protein as a Potential Molecular Indicator in Prostate Cancer.

ABSTRACT: Extracellular vesicles (EVs) are lipid nano-to-micro-sized vesicles increasingly studied for their role in intercellular communication and their potential as minimally invasive molecular indicators in various diseases. However, challenges remain in characterizing specific surface molecules on EVs due to cargo heterogeneity and the lack of convenient quantification methods. In this study, we show the isolation, characterization, detection, and quantification of Trop2-carrying EVs (EV-Trop2) in serum of prostate cancer patients. This work combines the unique advantages of our EV isolation method with ELISA to enable surface-protein-specific EV analysis directly from serum. This is, to our knowledge, the first demonstration to isolate and quantify EV-Trop2 from prostate cancer patient serum t

SUBMITTER: Sinawang PD 

PROVIDER: S-EPMC12456249 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature