Spatial effects of infiltrating T cells on neighbouring cancer cells and prognosis in stage III CRC patients.

ABSTRACT: Colorectal cancer (CRC) is one of the most frequently occurring cancers, but prognostic biomarkers identifying patients at risk of recurrence are still lacking. In this study, we aimed to investigate in more detail the spatial relationship between intratumoural T cells, cancer cells, and cancer cell hallmarks as prognostic biomarkers in stage III colorectal cancer patients. We conducted multiplexed imaging of 56 protein markers at single-cell resolution on resected fixed tissue from stage III CRC patients who received adjuvant 5-fluorouracil (5FU)-based chemotherapy. Images underwent segmentation for tumour, stroma, and immune cells, and cancer cell 'state' protein marker expression was quantified at a cellular level. We developed a Python package for estimation of spatial proximity, neare

SUBMITTER: Azimi M 

PROVIDER: S-EPMC12456411 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature