A simple seed-piercing transformation protocol for pearl millet and finger millet.

ABSTRACT: Pearl millet and finger millet face challenges in adopting transgenic or editing approaches due to their inherent recalcitrance to genetic transformation protocols. To overcome these limitations, the current study aims to streamline the genetic transformation protocol for pearl millet and finger millet. We targeted mature embryos as explants to assess transformation efficiencies, gain in time, and overall feasibility. Notably, a transformation efficiency of 17.74% and 18.79%, for pearl millet and finger millet, respectively, was observed using a method that involved directly piercing the mature seeds at the embryonic region with a needle dipped in Agrobacterium suspension, followed by vacuum infiltration. After infection, the seeds were allowed to produce calli and differentiate int

SUBMITTER: Moin M 

PROVIDER: S-EPMC12456579 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature