Improving vascular retention of indocyanine green for <i>in vivo</i> two-photon microscopy using liposomal encapsulation.

ABSTRACT:

Significance

Two-photon microscopy is widely used for in vivo imaging of vasculature in rodents and requires the labeling of blood plasma with fluorescent dyes such as indocyanine green (ICG). However, a major limitation of ICG is its rapid clearance from the body, which restricts its use in extended imaging sessions. We address and overcome that limitation, enabling longer in vivo imaging sessions.

Aim

We aim to investigate the feasibility of using liposomal nanoparticles that, when used to encapsulate ICG, significantly increase the circulation time of the vascular label in the rodent body.

Approach

We conducted in vivo imaging experiments with unencapsulated (free) ICG and liposomal ICG (L-ICG) and compared the retention of ICG in the vascular

SUBMITTER: Tomar A 

PROVIDER: S-EPMC12456867 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature