Quantification of Alzheimer disease neuropathology using tissue microarrays.

ABSTRACT: Alzheimer's disease (AD) is characterized by the hallmark pathologies of β-amyloid plaques and neurofibrillary tangles (NFTs), which vary across brain regions and between affected individuals. As a rapid research method to quantify hallmark pathologies and their relationships with the surrounding cytoarchitecture across the brain, we trialed tissue microarrays (TMAs) in combination with multiplex immunofluorescence and semi-automated image analysis. We assayed the hallmark pathologies across grey and white matter sites of 11 different brain regions and quantified astrocytes, microglia, and neurons in four AD cases and four age- and gender-matched controls. The results demonstrated that 2 mm cores differentiated AD-affected individuals from healthy donors. Regional amyloid loads corroborate

SUBMITTER: Nguyen-Hao HT 

PROVIDER: S-EPMC12456884 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature