One-carbon metabolism modulates miR-29a-DNA methylation crosstalk in Alzheimer's disease.

ABSTRACT:

Introduction

Alzheimer's disease (AD)'s multifactorial nature stresses the role of epigenetics in affecting different pathological pathways. We demonstrated that one-carbon metabolism epigenetically impacts AD-like phenotype. Here, we investigated the crosstalk between methylation and microRNAs in AD.

Methods

We altered one-carbon metabolism to induce hypo- and hyper-methylation, in SK-N-BE neuroblastoma cells and TgCRND8 mice. miRNAs were profiled through a polymerase chain reaction array, then we focused on miR-29a expression and methylation of its genomic locus. Finally, we assessed miR-29a expression and methylation in the brain of AD subjects.

Results

MiR-29a was repressed in hypomethylating and expressed in hypermethylating conditions. The expression of miR-29a

SUBMITTER: Raia T 

PROVIDER: S-EPMC12457075 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature