Phenotypes of synovial fluid Treg cells in checkpoint blockade-related inflammatory arthritis.

ABSTRACT:

Objectives

Regulatory T (Treg) cells may become dysregulated in checkpoint blockade-related inflammatory arthritis (CBIA), and we aimed to profile phenotypes and cytokine-secreting patterns of Tregs in CBIA.

Methods

Using a 77-protein panel, we here profiled and compared single-cell membrane proteomics of Treg cells in synovial fluid (SF) in 15 patients with active CBIA onset, 12 patients with active rheumatoid arthritis (RA), and 9 CBI-treated cancer patients with non-autoimmune inflammatory knee swelling. Microbead-sorted Treg subsets from CBIA patients underwent 32-cytokine panel secretome analysis. Peripheral blood (PB) Tregs from seven CBIA and six RA patients were similarly analyzed. Findings were correlated with the modified Clinical Disease Activity Index (mCDAI) in

SUBMITTER: He H 

PROVIDER: S-EPMC12457161 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature