Integrative Single-Cell and Machine Learning Analysis Reveals Immune Microenvironment Remodelling in Lymph Node Metastasis of Lung Adenocarcinoma.

ABSTRACT: Lymph node metastasis is a pivotal determinant of prognosis in lung adenocarcinoma, yet its impact on tumour microenvironment remodelling remains insufficiently characterised. In this study, we employed single-cell RNA sequencing to compare metastatic and non-metastatic lymph nodes, delineating metastasis-associated immune and stromal alterations. Metastatic nodes exhibited marked reductions in dendritic cell and T cell infiltration alongside increases in monocytes and SPP1⁺ macrophages, indicative of an immunosuppressive milieu. Intercellular communication analysis revealed strengthened interactions among SPP1⁺ macrophages, monocytes, and epithelial cells, suggesting coordinated signalling that may further enforce immune suppression. Integrating differentially expres

SUBMITTER: Jiang S 

PROVIDER: S-EPMC12457218 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature