Investigation of co-existing bacteria in platelets by employing long-term culturomics and metagenomics.

ABSTRACT:

Introduction

Bacterial contamination of platelets presents a substantial risk in transfusion medicine. Conventional detection approaches have limitations in sensitivity and bacterial coverage. In this study we employed culturomics and metagenomics to investigate co-existent bacteria in platelets, aiming to enhance transfusion safety and explore healthy bacteremia.

Methods

Platelet from 6 healthy donors underwent a 30-days extensive cultivation and isolation procedure using in-house culturomics.

Results

16S rRNA sequencing identified 90 bacterial strains across 3 phyla, 5 classes, 5orders, 7 families, 9 genera, and 23 species. Metagenomics sequencing revealed greater microbial diversity, detecting an average of 3018 microbial species per sample. The bacteria concurrent

SUBMITTER: Wang Q 

PROVIDER: S-EPMC12457405 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature