Dual roles of EGO-1 and RRF-1 in regulating germline exo-RNAi efficiency in Caenorhabditis elegans.

ABSTRACT: RNA interference (RNAi) is widely used in life science research and is critical for diverse biological processes, such as germline development and antiviral defense. In the germline of Caenorhabditis elegans, exogenous RNAi (exo-RNAi), the RNA-dependent RNA polymerases EGO-1 and RRF-1 play redundant roles in facilitating small RNA amplification. However, their coordination during the regulation of exo-RNAi processes in the germline remains unclear. Here, we examine non-null mutants of the ego-1 gene and find that ego-1(S1198L) animals exhibit germline exo-RNAi defects with normal fertility, abnormalities in germ granules, and synthetic temperature-dependent sterility with rrf-1. The exo-RNAi defects in ego-1(S1198L) are partially restored by inhibiting hrde-1 and znfx-1. Germline exo-RNAi

SUBMITTER: Dejima K 

PROVIDER: S-EPMC12457629 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature