<i>Jianpi-Huayu-Jiedu</i> formula improves metabolic dysfunction-associated steatohepatitis through ameliorateing hepatic oxidative stress and lipid accumulation <i>in vivo</i> and <i>in vitro</i>.

ABSTRACT: Hepatic oxidative stress and lipid accumulation are two key features of metabolic dysfunction-associated steatohepatitis (MASH). Jianpi-Huayu-Jiedu Formula (JPD) has been shown to ameliorate oxidative stress and lipid accumulation, suggesting its potential as an effective treatment for MASH. The current study aimed to explore the therapeutic effects of JPD on MASH. Mice fed a methionine- and choline-deficient (MCD) diet and HepG2 cells induced by oleic acid and palmitic acid (OA&PA) or lipopolysaccharide (LPS) were used as in vivo and in vitro models, respectively. Network pharmacology, molecular biology, and transcriptomics were employed to investigate the efficacy and mechanisms of JPD. Network pharmacology analysis indicated that the PI3K-AKT signaling pathway is a potenti

SUBMITTER: Wu X 

PROVIDER: S-EPMC12457829 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature