Glutaminase Expression in Canine Large-Cell Alimentary Lymphoma Cells and Effects of Glutaminase Inhibition by CB-839.

ABSTRACT: Glutamine metabolism plays a crucial role in tumour progression, making glutaminase a promising therapeutic target in various human cancers. However, its role in canine large-cell alimentary lymphoma (AL) remains unclear. This study investigated glutaminase expression and the effects of a glutaminase inhibitor (CB-839) on canine large-cell AL cell lines. Western blotting analysed glutaminase expression in three canine large-cell AL cell lines (CLC, Ema and Nody-1) and peripheral blood mononuclear cells (PBMCs) isolated from eight clinically healthy dogs. Cell viability was determined in each cell line after treatment with varying concentrations (0-10 µM) of CB-839. Flow cytometry was used to analyse the cell cycle and assess annexin assays in each cell line following treatment with 1 µM of

SUBMITTER: Sakai K 

PROVIDER: S-EPMC12457854 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature